ORIGINAL RESEARCH ARTICLE
Evaluation of dynamic serum thiol-disulphide homeostasis in colorectal cancer
Received Date : 11 May 2019
Accepted Date : 03 Aug 2019
Doi: 10.1016/j.jons.2019.08.001 - Article's Language: EN
J Oncol Sci 5 (2019) 60e64
in recent years. Thiol and disulphide play an important role in cell signal mechanisms, antioxidant
protection, and detoxification. In this study, we aimed to evaluate the role of Thiol-Disulphide homeostasis
(TDH) in colorectal cancer (CRC) by using a new method.
Material and Method: The patients (pts) who diagnosed with CRC and healthy control subjects were
included to study. Serum samples for the thiol-disulphide test were obtained at the time of diagnosis.
TDH tests were measured by the automated spectrophotometric method by describing Erel and Neselio
glu. Thiol-disulphide homeostasis was also evaluated according to tumor stage and localization.
Results: Eighty-eight pts with CRC and 110 control were enrolled. Native thiol (NT), disulphide and total
thiol (TT) levels were significantly lower in patients compared with the control arm (Median NT: 402
e424, p ¼ 0.003; median Disulphide 18.7e21, p ¼ 0.011; median TT: 437e467, p ¼ 0.001). Thiol/disulphide
balance was also maintained (p ¼ 0.149). TT and NT levels were not differed according to tumor
localization whereas disulphide level was significantly higher in left-sided tumors than right-sided (19.9
e13.07 respectively, p ¼ 0.007). In addition, disulphide/NT ratio was also significantly higher in left-sided
than right-sided (0.1e0.12 respectively, p ¼ 0.015). Thus, the balance of dynamic TDH is disrupted in
favor of disulphide between left-sided and right-sided tumor. There was also no significant difference
between thiol-disulphide levels and tumor stage whereas thiol level tends to lower in stage 4 disease
(p ¼ 0.7).
Conclusion: This is the first trial that evaluates the relationship with dynamic TDH and CRC according to
tumor stage and localization. Thiol and disulphide may play an important role in the pathogenesis of
Keywords: Colorectal cancer; disulphide; oxidative stress; thiols