Bugi Ratno Budiartoa, Pimpin Utama Pohan b, Desriani a
a Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km. 46, Cibinong, 16911, Indonesia
b Faculty of Medicine, University of North Sumatra, Jalan Dr. T. Mansyur No.5, Padang Bulan, Medan Baru, Kota Medan, Sumatera Utara, Indonesia
Doi: 10.1016/j.jons.2018.12.001 - Article's Language: EN
Single Nucleotide Polymorphism at codon 655 of HER2 gene has been extensively evaluated for its role as
a susceptible biomarker for breast cancer development and the contradictive result of its role has been a
debate among researchers as evidenced from case-control studies. Three platforms of molecular
detection systems named PCR-RFLP, TaqMan assay, and AS-PCR have been used intensively in elucidating
this important SNP with considering the affordability and simplicity of detection especially in research
format which employs plenty of samples such as in the epidemiological study. Nevertheless, methodological
related-bias generated from the association study between HER2I655V SNP and breast cancer risk
becomes primary drawback that must be addressed seriously in an attempt to obtain a solid conclusion.
This review will discuss the application of nucleic acid amplification-based methods for HER2I655V SNP
detection, the potency of bias generated by these genotyping technologies, and strategies to improve
their reliability of detection.
Keywords: SNP; HER2I655V gene; Breast cancer; Genotyping methods; Genotyping errors